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KMID : 0043320010240020150
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Archives of Pharmacal Research
2001 Volume.24 No. 2 p.150 ~ p.158
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Inhibition of Heat-induced Denaturation of Albumin by Nonsteroidal Antiinflammatory Drugs(NSAIDS): Pharmacological Implications
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Saso L
Valetini G/Casini ML/Grippa E/Gatto MT/Leone MG/Silvestrini B
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Abstract
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The activity of nonsteroidal antiinflammatory drugs(NSAIDS) in rheumatoid arthritis is not only due to the inhibition of the production of prostaglandins, which can even have be nrficial immunosuppressibe effects in chronic inflammatory processes. Since we speculated that these drugs could also act by protectin endogenous proteins against denaturation, we ebaluated their effect on heat-induced denaturation human serum albumin in comparison with several fatty acids which are known to be potent stabilzers of this protein. By the Mizushimas assay and a recently developed HPLC method was less were slightly less active[EC50~10-5-10M] than the HPLC method was less sensitive but more selective than the turbidimetric assay, I.e. it was capable of distinguishing true antiaggregant agents like FA and NSAIDs from substances capable of inhibiting the precipitation of denatured protein aggregates. In conclusion, this survey could be useful for the development of more effective agents in protein condensation diseases like rheumatic disorders, cataract and Alzheimers disease.
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